Automatic sampler vial cap septum problems Contamination can occur from vial cap septa, particularly if more than three injections are made from one vial. Repeated punctures may dislodge septum pieces into the sample. To determine if this is a contamination source, cut a 1- x 2-mm segment from a septum and slurry in a vial with 1 ml of the same
Vials are processed and tested for background particulates. A typical vial that has not been processed can exhibit particle counts exceeding 5000 particles per mL with the highest counts occurring in the range below 0.5µm. This has traditionally been of little concern when GC inlet liners or HPLC guard columns are used. GC techniques
Nov 10, 2022 · Vials. Use only Aijiren vials, the shape, and the material is optimized for maximizing instrument precision. Do not overfill the vials. Fill the vial up to 1/2 to 2/3 of the total volume. Experimental conditions. Make sure that the same solvents were used between the runs. Keep the room temperature constant and under 25 degrees Celsius.
Nov 10, 2022 · Set the active method vial size to 20 mL. Press [Vial], scroll to Vial size, Press [Mode/Type] to select the vial size from a list, then press [Enter]. Install a new septum into the blue leak test vial. Place the blue leak test vial into priority position 1 (111 vial model) or into tray position 1 (12 vial model). Set the carrier pressure to a
Nov 10, 2022 · Contamination that creates ghost and carry over peaks can be introduced at different points including sample preparation, introduction, and separation. The additional peaks can cause false positive identifications and/or inaccurate quantitative results. Other chromatographic problems are identified in Basic Troubleshooting for GC Systems
Sep 21, 2004 · Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques. Parafilm Contamination? (HPLC) We use parafilm extensively throughout our lab. we are seeing erratic retention times and basline drifts. Our mobile Phases are mainly buffered solutions without organics, however our needle rinse and seal wash are 90% methanol
Feb 27, 2023 · The vial can crack or break, leading to sample contamination or loss. 2. Chemical attack: Some samples or solvents used in HPLC analysis can be aggressive and may react with the vial material
Jan 13, 2017 · Abstract. Cross-contamination from the polymeric material of the vial caps used in size exclusion chromatography (SEC) and high-performance liquid chromatography autosamplers in the low molar mass (LMM) range (<2000 Da) of polymer samples was demonstrated with the help of matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) analysis.
3. Sample contamination – sharp ghost peaks tend to indicate contamination of sample, and can arise from many sources and from various stages in sample preparation. To avoid, you should eliminate sources of contamination from your sample preparation protocol and ensure that you are using clean vials . 4.
May 10, 2023 · Clear vials can accommodate a wider variety of solvents than amber vials due to the absence of iron oxide, which reacts with certain solvents and leads to the contamination of samples. Clear vials
transfer. These issues, as well as other instrument setup-related topics, are addressed later in the System Optimization section of this guide. Samples must be prepared to maximize the concentration of the volatile components in the headspace, and minimize unwanted contamination from other compounds in the sample matrix.
Resolving IC analysis issues Ion Chromatography (IC) is the premier technique for determining ionic compounds in solution. The basic components consist of an eluent source, pump, sample injector, separating column, suppressor, and detector. As with any lab instrumentation, your IC system should be serviced regularly to ensure peak performance.
General chromatography vials: Septa material In this section, the key attributes to the different septa available can be found. PTFE/natural red rubber PTFE/natural red rubber are moderately priced seals for GC and HPLC with good chemical properties. They are ideal for multiple injections due to high resealability, but not as easy to penetrate as
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What Are Common Peak Shape Issues? 1. Split peaks 2. Peak tailing 3. Broad peaks • Many peak shape issues are also combinations - i.e. broad and tailing or tailing with increased retention •Symptoms do not necessarily affect all peaks in the chromatogram •Each of these problems can have multiple causes Page 12