The minimum gas flow for direct injection is about 5 mL/min. Direct injection can therefore only be applied to columns, which can cater for higher column flow rates such as, packed columns or 0.53 mm ID columns. These columns can also handle the higher concentration levels because of their increased sample capacity.
Jun 27, 2023 · In general, modern chromatography liquid autosamplers accept 12 x 32mm vials, so we will assume that a 12 x 32mm vial is compatible with your autosampler (Headspace autosamplers accept 20mm wide
Consumables (GC-, LC-Columns, Sample Prep Cartridges, Vials, Syringes, etc.) Choosing the right pore size for size exclusion chromatography. ... Peptide Mapping ...
Guidelines for sample preparation: Use appropriate solvents for the ESI-MS system. a. Appropriate solvents include: • Acetonitrile. • Methanol. • Water. b. If your compound is not sufficiently soluble in one of the above, you can first dissolve it in DMSO, but you must dilute the DMSO to 0.1%.
Aug 31, 2022 · Two types of applications: The most common vial sizes for liquid chromatography vials are 12 x 32 mm as well as 15 x 45 mm. 12 x 32 mm vials will also be described as 1.5 mL, 1.8 mL, or 2.0 mL
For example, low-quality caps can result in blocked needles or needle seats due to coring. Maintenance tips. Ensuring continual maintenance can help with troubleshooting HPLC autosamplers, and should be done at least every 12 months. Components to consider include: - Needle and needle seat.
Replace derivatization reagent (OPA and FMOC), borate buffer, and amino acid standards daily. The FMOC and OPA reagents are especially fragile, so the content of each ampule should be transferred into vials with inserts and refrigerated. The FMOC and OPA can be used up to 5 days after opening the ampule.
Overview. Available in LC-GC, LC-MS, and TruView pH Control LCMS Certified Vials with cleanliness tested by UPLC-UV and MS. Consistent vial-to-vial and lot-to-lot performance. High recovery vials for limited sample volumes. Compatible with all autosamplers and designed to fit a range of caps. Complete selection of vial sizes and materials with
Mar 17, 2022 · 1000Pcs 2ml Autosampler Vials with Writing Area and Graduations, 9-425 HPLC, Blue ABS Screw Caps & Septa for GC Sample Vials Brief content visible, double tap to read full content. Full content visible, double tap to read brief content.
Pump Right flow rate: 0.400 mL/min Pump Left flow rate: 1 mL/min DAD settings: UV_1: 214 nm UV_ 2: 280 nm, 3D Field on (200-300 nm) Corona ultra RS settings: Filter setting: 4 Nebulizer temp: 15 °C Power function: 1.0 Pump Right was used for the SEC analysis and ran isocratically with 10 mM ammonium acetate, pH 6.7 with 5% acetonitrile.
Aijiren Technology nano- and microflow LC-MS columns are specifically designed for low-dispersion nano LC systems. The selected stationary phases of these analytical columns fully leverage the separation power of sub-2–µm particle technology at flow rates between 100 nL/min and 50 µL/min, withstanding 15,000 psi, and have an inner diameter between 75
Vial Mouth Opening A 6mm opening for the 1.5mL vial provides a better target for the autosampler needle and makes filling easier and cleaner, especially with automatic pipettors. LabTotal vials have a 9mm opening for maximum sample retrieval via pipette. Glass Thickness Uniformity of the vial bottom thickness ensures consistent needle
The approach taken in our AdvanceBio Peptide Plus columns is to chemically modify the silica surface to block the residual silanols and repel positively charged samples. This allows us to get nice sharp peaks and good peak capacity for peptide mapping with formic acid mobile phases for MS detection. I want to wrap up with a bit of a disclaimer.
TruView Vials exhibit the lowest surface adsorption of any LC autosampler vial on the market. 96-Well Plate, 700 µL per wellis made from a clean grade of polypropylene. The wells are designed to maximize sample recovery for all ACQUITY UPLC®Systems.