The final mAb concentration in each vial was 0.2 mg/mL. Each test condition was aliquoted into 3 mL glass vials, 2.5 mL per vial, in a clean laminar flow hood. Four vials were prepared per timepoint per condition. Vials were stored at 2–8°C and 40°C for 2 weeks. The analytical testing included SDS-PAGE, cIEF, and MS analyses.
Jan 1, 2020 · Type I glass, the most used primary packaging material in vials, and a relatively inert one, can adsorb relatively large amounts of proteins during the shelf life of a drug, mostly due to electrostatic interactions. 84 Glass and silica microparticles have also been shown to cause adsorption of humanized mAbs to their surface, in about 4-nm
Apr 1, 2020 · For example, small-scale studies can generate data on residual VHP in isolator air (i.e., VHP profile) and other product contact materials (silicone tubing, vials, etc.), thus allowing one to
May 10, 2016 · May 10 2016. Supelco’s Low Adsorption (LA) vials are manufactured using a process that decreases the number of hydroxyl groups on the vial's glass surface, significantly reducing surface activity while improving analytical quantitation and minimising pH shifts in the sample. This same process also removes unwanted surface metals such as
Part 1 gave an outline of mechanisms of sample adsorption to containers and how to reduce this adsorption. Part 2 describes the characteristics of LabTotal Vial (glass) and TORAST™-H Bio Vial (polypropylene) low-adsorption vials developed based on mechanisms of container adsorption, and the adsorption-reducing effect of these vials on basic
The Chapters are further organized by sample types so that gaseous samples are first (Chapter 6) followed by liquid samples (or samples put into a liquid form) (Chapters 7-12), solid samples (Chapter 14), biological samples (nucleic acids and proteins)(Chapter 15), biological fluids/tissues (Chapter 16) and then special topics such as sample
1) The low-adsorption glass vials (LabTotal vials) inhibit the adsorption of basic compounds. 2) The low-adsorption PP vials (TORAST-H TM Bio vials) inhibit the adsorption of peptides. These low-adsorption vials are expected to help ensure the reliability of high-sensitivity analysis.
This results in extremely low compound loss through adsorption or catalytic degradation. Our glass vials have very low reactivity, pH shift, and leaching characteristics. Extra Low Adsorption vials are a must have for MS users or when working with micromolar concentrations and/ or low sample volumes, where the surface-to-volume effects are much
Aug 18, 2021 · where \( \frac{dq}{dt} \) is the heat transfer rate in Joule·h −1, K v is the vial heat transfer coefficient in Joule·h −1 ·cm −2 ·°C −1, A v is the outer area of the vial in cm 2, T s is the temperature of the shelf surface in °C, T b is the temperature of the product in contact with the bottom of the vial in °C, \( \frac{dm}{dt} \) is the mass flow rate in g·h −1, and ∆H
The analysis of low abundance species, pH sensitive analytes or not yet well understood compound mixtures benefit from consistent and well controlled vial surfaces. The glass surface of TruView pH Control LCMS Certified Vials minimizes pH shifts away from the neutral and thus eliminates unwanted reactions and resulting ghost peaks.
Sep 30, 2021 · Solid phase extraction (SPE) plays an important role in chemical and biological analysis. Compared with liquid–liquid extraction, SPE has the advantages of low solvent consumption, high efficiency, low cost, convenient operation and short time-consuming. However, the adsorbent used in SPE usually shows poor selectivity. The molecularly imprinted polymers (MIPs), which is similar to the
The analysis of low abundance species, pH sensitive analytes or not yet well understood compound mixtures benefit from consistent and well controlled vial surfaces. The glass surface of TruView pH Control LCMS Certified Vials minimizes pH shifts away from the neutral and thus eliminates unwanted reactions and resulting ghost peaks.
I have focused on the development of versatile low-adsorption vials suitable for use in HPLC auto samplers, where the surface finish of the container is optimized to reduce ionic and hydrophobic sample adsorption.
Jun 22, 2020 · Tricyclic antidepressants (TCAs) were prepared in serum using five different glass vials with the goal of finding a vial type with least adsorption and good vial-to-vial reproducibility. LC-mass spectrometry (MS) results were obtained 0, 2, 4, 12 and 24 hours after filling up the vial.
Jun 3, 2021 · To study the adsorption characteristics of CO, CO 2, N 2, O 2, and their binary-components in lignite coal, reveal the influence of CO 2 or N 2 injection and air leakage on the desorption of CO in